Daim ntawv thov ntawm ultrafiltration hauv protein sib cais thiab purification

Ultrafiltration technology yog ib tug tshiab thiab high-efficiency sib cais tshuab.Nws muaj cov yam ntxwv ntawm cov txheej txheem yooj yim, muaj txiaj ntsig zoo hauv kev lag luam, tsis muaj theem hloov pauv, sib cais loj coefficient, txuag hluav taws xob, kev ua haujlwm siab, tsis muaj kuab paug theem nrab, kev ua haujlwm tas li ntawm chav tsev kub thiab lwm yam.Niaj hnub no, tus thawj coj Yang uas los ntawm Beijing nug txog peb cov khoom siv ultrafiltration rau kev ntxuav cov protein thiab sib txuas lus nrog peb cov thev naus laus zis.Tam sim no, tus editor ntawm Shandong Bona pawg yuav qhia txog daim ntawv thov ntawm ultrafiltration nyob rau hauv protein sib cais thiab purification.

1. Rau protein desalination, dealcoholization thiab concentration
Cov ntawv thov tseem ceeb tshaj plaws ntawm ultrafiltration hauv kev ua kom huv ntawm cov protein yog desalting thiab concentration.Ultrafiltration txoj kev rau desalination thiab concentration yog yus muaj los ntawm loj batch ntim, lub sij hawm ua hauj lwm luv luv thiab high efficiency ntawm protein rov qab.Cov txheej txheem ntawm steric exclusion chromatography kom tshem tawm ntau yam tshuaj los ntawm cov proteins tau raug hloov los ntawm niaj hnub ultrafiltration technology, uas tau dhau los ua cov thev naus laus zis tseem ceeb rau cov protein desalination, dealcoholization thiab concentration hnub no.Nyob rau hauv xyoo tas los no, thev naus laus zis ultrafiltration tau siv dav hauv kev desalination thiab rov ua kom muaj txiaj ntsig zoo ntawm cov protein ntau hauv cheese whey thiab soybean whey.Lactose thiab ntsev thiab lwm yam khoom xyaw hauv cov protein, nrog rau cov kev xav tau tiag tiag ntawm kev ua tiav cov desalting, de-alcoholization thiab concentration ntawm cov protein.Kev siv thev naus laus zis ultrafiltration tuaj yeem mloog zoo rau serospecies immunoglobulins kom tau raws li qhov xav tau ntawm cov protein ntau.

2. Rau cov protein fractionation
Protein fractionation yog hais txog cov txheej txheem ntawm kev sib cais ntawm txhua qhov sib xyaw ntawm cov protein los ntawm ntu raws li qhov sib txawv ntawm lub cev thiab tshuaj lom neeg (xws li tus txheeb ze molecular hnyav, isoelectric point, hydrophobicity, thiab lwm yam) ntawm txhua cov protein tivthaiv hauv cov kua pub.Gel chromatography yog ib txoj hauv kev siv ntau tshaj plaws rau kev faib cov macromolecules lom (tshwj xeeb tshaj yog cov protein).Piv nrog rau cov tsoos chromatography, ultrafiltration kev sib cais tshuab muaj kev cia siab zoo ntawm daim ntawv thov nyob rau hauv lub fractionation thiab industrial zus tau tej cov proteins thiab enzymes nrog ib tug tseem ceeb economic nqi vim hais tias ntawm nws cov nqi qis thiab yooj yim amplification.Qe dawb yog cov khoom siv pheej yig tshaj plaws kom tau txais lysozyme thiab ovalbumin.Tsis ntev los no, ultrafiltration feem ntau yog siv los cais ovalbumin thiab lysozyme los ntawm qe dawb.

3. Kev tshem tawm Endotoxin
Kev tshem tawm Endotoxin yog ib qho ntawm cov ntawv thov tseem ceeb ntawm kev siv tshuab ultrafiltration hauv protein purification.Cov txheej txheem tsim endotoxin yog qhov nyuaj heev.Nyob rau hauv cov txheej txheem ntawm kev siv tswv yim, vim hais tias cov tshuaj protein ua los ntawm prokaryotic qhia system yog ib qho yooj yim mus sib tov nrog endotoxin uas ua los ntawm cov kab mob cell phab ntsa tawg, thiab endotoxin, los yog hu ua pyrogen, yog ib hom lipopolysaccharide.Tom qab nkag mus rau tib neeg lub cev, nws tuaj yeem ua rau kub taub hau, microcirculation cuam tshuam, endotoxic shock thiab lwm yam tsos mob.Txhawm rau tiv thaiv tib neeg kev noj qab haus huv, nws yog ib qho tsim nyog yuav tsum siv tshuab ultrafiltration kom tshem tawm endotoxins.

Txawm hais tias siv tshuab ultrafiltration yog dav siv hauv kev sib cais thiab kev ua kom huv ntawm cov protein, nws kuj muaj qee yam kev txwv.Yog tias qhov hnyav molecular ntawm ob cov khoom yuav tsum tau sib cais tsawg dua 5 zaug, nws tsis tuaj yeem raug cais los ntawm ultrafiltration.Yog tias qhov hnyav molecular ntawm cov khoom yog tsawg dua 3kD, nws tsis tuaj yeem ua kom pom tseeb los ntawm ultrafiltration, vim tias ultrafiltration feem ntau ua rau qhov hnyav ntawm qhov hnyav ntawm daim nyias nyias ntawm 1000 NWML.

Nrog rau kev txhim kho txuas ntxiv ntawm kev lag luam bioengineering, cov kev xav tau siab dua tau muab tso rau pem hauv ntej rau kev sib cais downstream thiab purification technology.Cov txheej txheem ntawm lub tshuab nqus tsev concentration, hnyav rho tawm, lim ntshav, centrifugation, nag lossis daus thiab tshem tawm pyrogen tsis muaj peev xwm ua tau raws li qhov xav tau ntawm ntau lawm.Ultrafiltration thev naus laus zis yog khi rau ntau thiab siv ntau dua vim nws qhov zoo ntawm kev sib cais protein.